Table 2.

List of primers used for the identification of Staphylococcus aureus based on 16 rRNA gene locus sequencing and for detection of four toxin encoding genes involved in food-poisoning, e.g., sea, sed, see, and seg.

Gene	Primer	Sequence (5′–3′)	Reference
Species identification
16S rRNA	27 F	AGA GTT TGA TCM TGG CTC AG	Lane, 1991
	1492 R	TAC GGY TAC CTT GTT ACG ACT T	Turner et al., 1999
Toxin
sea	SA-U	TGTATGTATGGAGGTGTAAC	Sharma et al., 2000
	SA-A	ATTAACCGAAGGTTCTGT	Sharma et al., 2000
sed	SED-1	GAATTAAGTAGTACCGCGCTAAATAATATG	Jarraud et al., 2002
	SED-2	GCTGTATTTTTCCTCCGAGAGT	Jarraud et al., 2002
see	SEE-1	CAAAGAAATGCTTTAAGCAATCTTAGGC	Jarraud et al., 2002
	SEE-2	CACCTTACCGCCAAAGCTG	Jarraud et al., 2002
seg	SEG-1	AATTATGTGAATGCTCAACCCGATC	Jarraud et al., 2002
	SEG-2	AAACTTATATGGAACAAAAGGTACTAGTTC	Jarraud et al., 2002