Figure 5.

Zebrafish Caiap is required for the inflammasome-dependent resistance to S. Typhimurium. (A) Scheme showing the experimental procedure used for the caspase-1 activity assays. Zebrafish one-cell embryos were injected with MOs and/or mRNAs, dechorionated and infected at 2 dpf via the yolk sac with ST with a MOI of 50, and collected and pooled (25–35 larvae) at 24 hpi to measure caspase-1 activity. (B) Scheme showing the experimental procedure used for the survival assays. Zebrafish one-cell embryos were injected with MOs and/or mRNAs, dechorionated and infected at 2 dpf via the yolk sac with ST at a MOI of 10, and the number of surviving larvae counted daily during the next 5 days. At least three independent experiments were performed with a total number of 300 specimens/treatment. (C–F) Zebrafish one-cell embryos were injected with standard control (Std) (C–F) or Caiap MOs (C–F), or with antisense (As) (C–F), Caiap (C,D) or CaiapΔCARD (E,F) mRNAs, infected at 2 dpf with wild-type ST and the caspase-1 activity (C,E) and survival (D,E,G) were determined as described in Figures 5A,C, respectively. The sample size for each treatment is 30 for (C,E), and 300 for (D,F,G). S.I., ST infection. **p < 0.01; ***p < 0.001.