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. 2017 Oct 19;8:1375. doi: 10.3389/fimmu.2017.01375

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Copyright © 2017 Tyrkalska, Candel, Pérez-Oliva, Valera, Alcaraz-Pérez, García-Moreno, Cayuela and Mulero.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The type 3 secretion system and flagellin of S. Typhimurium act upstream Caiap. (A–F) Zebrafish one-cell embryos were injected with standard control (Std) or Caiap MOs, or with antisense (As) or Caiap mRNAs, infected at 2 dpf with wild-type (WT) (A-F), the double mutant SPI 1–2 (A–D), FliCON or FliC FljB ST (E,F) and the survival (A,C,E) and caspase-1 activity (B,D,F) were determined as described in Figures 5A,B, respectively. The sample size for each treatment is 300 for (A,C,E), and 30 for (B,D,F). S.I., ST infection; ns, not significant; *p < 0.05; **p < 0.01; ***p < 0.001.