Figure 2.

Presence of Siglec-1^hiCD4⁺MDM dampens the degree of human immunodeficiency virus type 1 (HIV-1) infection. Monocytes from three donors were differentiated into monocyte-derived macrophage (MDM) with M-CSF media. (A) Panels show plots of unfractionated MDM and their Siglec-1^hiCD4⁺MDM-depleted counterparts. (B) Unfractionated MDM and Siglec-1^hiCD4⁺MDM-depleted cultures were infected with purified HIV-1 (BaL, JRFL, or US-1). Cells were harvested on day 3 postinfection, stained, and analyzed by flow cytometry. Panels show plots of HIV-1 infection in the unfractionated MDM cultures and in their Siglec-1^hiCD4⁺MDM-depleted counterparts. Values in the upper right quadrant(s) represent the percentage of infected MDM. Data represent one of the triplicate wells from one of two independent experiments. (C) Bar graph is derived from the experiment performed in Figure 2B and includes the data from the triplicate wells of two independent experiments and shows the% p24⁺MDM (mean ± SD) in unfractionated MDM (filled bars) or in the Siglec-1^hiCD4⁺MDM-depleted MDM (open bars) following infection with HIV-1. Significance is indicated by *p ≤ 0.05.