Figure 2.

Knockdown or overexpression of LASS2 in EJ-M3 cells impact tumor growth in nude mice. EJ-M3 cells were transfected with a LASS2-over plasmid or a LASS2 shRNA plasmid, and then injected subcutaneously into the right dorsal aspect of the neck of nude mice. Non-infected EJ-M3 cells were used as the Con. The (A) tumor volumes and (B) body weights of mice were measured weekly until 6 weeks following injection. (C) Images of nude mice were taken 6 weeks following injection. (D) A total of 6 weeks following injection, all mice were sacrificed, and the tumors were resected and assessed. (E) Tumor volumes were measured following tumor resection. *P<0.05, **P<0.01 vs. the Con group. LASS2, LAG1 longevity assurance homolog 2; Con, control; LASS2-over, LASS2 overexpression group; shRNA, short hairpin RNA.