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. Author manuscript; available in PMC: 2017 Oct 31.

Published in final edited form as: Chem Rev. 2017 May 12;117(12):7857–7877. doi: 10.1021/acs.chemrev.7b00046

Dynamics of PCNA on a UV-damaged template. During DDT, the retention of PCNA at a blocked P/T junction abutting a UV-induced lesion ( ) is governed by three activities; 1) Enzymatic loading of PCNA onto the P/T junction; 2) Enzymatic unloading of PCNA from the P/T junction and; 3) diffusion of PCNA along either the nascent dsDNA or the adjacent ssDNA. If PCNA can vacate a blocked P/T junction, limiting PCNA rings will not be continuously re-loaded. However, diffusion of PCNA along DNA and enzyme-catalyzed unloading PCNA from DNA are prohibited during TLS, promoting retention of PCNA at blocked P/T junctions during S-phase.

Inline graphic — Dynamics of PCNA on a UV-damaged template. During DDT, the retention of PCNA at a blocked P/T junction abutting a UV-induced lesion ( ) is governed by three activities; 1) Enzymatic loading of PCNA onto the P/T junction; 2) Enzymatic unloading of PCNA from the P/T junction and; 3) diffusion of PCNA along either the nascent dsDNA or the adjacent ssDNA. If PCNA can vacate a blocked P/T junction, limiting PCNA rings will not be continuously re-loaded. However, diffusion of PCNA along DNA and enzyme-catalyzed unloading PCNA from DNA are prohibited during TLS, promoting retention of PCNA at blocked P/T junctions during S-phase.