Fig. 4.

MiR-200b target MAPK7 to inhibit TGF-β-induced EMT and tumor proliferation. a The potential binding site of miR-200b in 3′-UTR of MAPK7 was predicted. HCCC cell-line was transfected with full-length 3′-UTR (wild-type or mutant) of MAPK7, and luciferase reporter was performed to confirm the direct target sites. b-c HCCC cells were overexpressed with miR-200b and MAPK7 and the mRNA and protein expression of E-cadherin, TTF-1, fibronectin, and α-SMA were determined by Q-PCR and western blot. d-e Transwell assay was performed to determine migration and invasion. f HCCC cells were overexpressed with miR-200b or MAPK7, and the cell vitality and proliferation were measured by CCK-8. g The expression of cyclin D1 and CDK2 were assessed by western blot