Table 4.

Analysis of consumed substrate and the production of anaerobic metabolites after 20 h of cultivation in a serum bottle for wild-type IAM1183 and mutants (n = 3)

End product	Strain
	IAM1183	IAM1183/R	IAM1183/N	IAM1183-CD	IAM1183-CD/R	IAM1183-CD/N	IAM1183-CDE	IAM1183-CDE/R	IAM1183-CDE/N
Glucose consumed (mM)	57.42 ± 1.38	59.27 ± 0.69	60.62 ± 0.85	70.02 ± 0.66	68.13 ± 0.94	74.05 ± 0.43	69.02 ± 0.77	69.28 ± 0.49	71.09 ± 0.39
Residual sugar (mM)	4.66 ± 0.25	4.33 ± 0.12	4.09 ± 0.15	2,4 ± 0.12	2.74 ± 0.17	1.67 ± 0.08	2.58 ± 0.14	2.53 ± 0.09	2.2 ± 0.07
2,3-Butanediol (mM)	9.35 ± 1.56	11.86 ± 0.91	11.53 ± 0.78	11.73 ± 1.37	24.75 ± 0.42	13.79 ± 1.09	8.26 ± 0.17	11.96 ± 0.78	10.69 ± 0.26
Lactate (mM)	23.62 ± 0.46	44.15 ± 0.99	31.54 ± 0.96	18.78 ± 1.03	25.33 ± 0.81	22.31 ± 0.66	32.14 ± 0.29	36.61 ± 0.45	38.21 ± 0.36
Acetate (mM)	17.27 ± 0.32	10.36 ± 0.39	20.31 ± 0.65	27.96 ± 0.69	23.17 ± 0.55	30.52 ± 1.26	17.16 ± 0.31	15.27 ± 0.13	16.01 ± 0.63
Formate (mM)	2.24 ± 0.17	3.92 ± 0.77	2.83 ± 0.98	6.19 ± 0.76	5.98 ± 0.47	8.28 ± 1.62	3.67 ± 0.34	2.92 ± 0.19	1.63 ± 0.27
Succinate	1.86 ± 0.08	1.01 ± 0.11	2.31 ± 0.19	3.22 ± 0.17	3.02 ± 0.12	3.59 ± 0.36	3.79 ± 0.48	3.02 ± 0.12	3.86 ± 0.23
Ethanol (mM)	31.93 ± 1.2	13.85 ± 2.02	38.09 ± 0.98	38.69 ± 1.16	14.47 ± 1.32	40.87 ± 0.72	25.14 ± 0.26	23.49 ± 1.19	29.96 ± 0.77
Citric acid (mM)	1.68 ± 0.25	1.52 ± 0.49	1.43 ± 0.33	1.69 ± 0.37	1.51 ± 0.72	1.65 ± 0.96	1.57 ± 0.67	1.62 ± 0.22	1.64 ± 0.93
Acetoin (mM)	25.71 ± 1.11	19.21 ± 0.58	27.32 ± 0.64	20.99 ± 0.84	20.13 ± 0.74	25.02 ± 0.17	23.19 ± 0.29	26.81 ± 0.44	25.62 ± 0.51
H2 yield (mol/mol glucose)	1.47 ± 0.06	1.52 ± 0.13	1.56 ± 0.17	1.83 ± 0.09	1.67 ± 0.41	1.99 ± 0.22	2.14 ± 0.69	2.08 ± 0.12	2.28 ± 0.12