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. Author manuscript; available in PMC: 2017 Oct 31.
Published in final edited form as: Methods Mol Biol. 2017;1567:155–178. doi: 10.1007/978-1-4939-6824-4_10

Fig. 2.

Fig. 2

MSP constructs available through the Addgene plasmid repository. The composition of each construct includes N-terminal hexahistidine affinity tags (6H), cleavage sites including Factor X (FX) and tobacco etch virus (TEV) proteolysis sequences, and apo-I derived helices comprising 11 residues (H1Δ, H3 and H9) and 22 residues (H1, H2, H4, H5, H6, H7, H8 and H10). The molecular weights indicated are for the intact, uncleaved polypeptide. The disc diameters are published values based on gel filtration chromatography and small angle x-ray scattering (Denisov et al., 2004).