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Nanodisc Affinity Purification Buffers
| Buffer | ND Column Buffer 1 Volume (ml) |
ND Column Buffer 2 Volume (ml) |
|---|---|---|
| Wash 1 (no imidazone) | 25.00 | 0 |
|
| ||
| Wash 2 (20 mM imidazole) | 23.75 | 1.25 |
|
| ||
| Wash 3 (50 mM imidazole) | 21.87 | 3.13 |
|
| ||
| Elution (400 mM imidazole) | 0 | 25.00 |
|
| ||
