Extended Data Figure 2. Resident MKs in lung and other organs.

(a) Survey of PF4-tomato mouse lung visualized by 2PIVM. PF4-tomato expressing cells (in red) are found in high numbers in the lung. Lung vasculature is labeled by intravascular injection of FITC dextran (green). A total area of 2.49 mm² (1.6 mm × 1.6 mm) was imaged. (b) Resident (static) GFP⁺ cells are found in PF4-mTmG (donor lung) to mTmG (recipient) transplanted mouse. (c) BM and (d) spleen 2PIVM images of PF4-mTmG mice. (c,d) Large-sized MKs (GFP, green) are found in the BM and spleen in large numbers. (e) Size characterization of resident (static) GFP⁺ cells by image analysis of PF4-mTmG lungs, BM, and spleen. Min-to-max boxplots are presented: the line in the middle of the box is plotted at the median, the box extends from the 25th to 75th percentiles and the whiskers range from the smallest to the largest values. The + indicates the mean.