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. Author manuscript; available in PMC: 2017 Oct 31.
Published in final edited form as: Nature. 2017 Mar 22;544(7648):105–109. doi: 10.1038/nature21706

Extended Data Figure 8. Lung hematopoietic progenitors are extravascular and have multi-lineage capabilities.

Extended Data Figure 8

(a) Representative spleen FACS plots of hematopoietic progenitors within the LSK compartment (LSK: Lin-,/Sca-1+/c-Kit+) and the myeloerythroid progenitors compartment (MP: Lin-/Sca-1-/c-Kit+). (b) Cell counts of hematopoietic progenitor populations in the spleen. (c-e) Lungs were perfused before digestion. (c) Representative FACS plot of LSK and MP compartments with antegrade perfusion +/- retrograde perfusion. (d) Frequencies and (e) cell counts of lung hematopoietic progenitor populations. (f-h) CD45-APC mAb was injected i.v. via the tail vein 5 min before lung digestion and staining with CD45-FITC mAb. (g) Hematopoietic progenitor populations were examined for labeling with injected CD45 mAb by flow cytometry. (h) Percentage of CD45-FITC+ cells positive or negative (extravascular cells) for the i.v. CD45-APC mAb. (i-k) Peripheral blood was analyzed 2-3 months after mTmG→c-mpl-/- lung transplants (i) Representative FACS plot of blood cell analysis. (j) Percentage of lung-derived cells (Tom+/CD41-) in the blood after transplant. (k) Percentage of B cells (CD19+), T cells (CD3+) or neutrophils (CD11b+, Ly6G+) in the lung-derived cells (Tom+/CD41-). Mean ± SEM are presented (n=5 mice per group).