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. 2017 Oct 16;127(11):4148–4162. doi: 10.1172/JCI83626

Figure 3. CB1 deletion in adipocytes affects caloric intake and EE and promotes alternative macrophage activation.

Figure 3

(A) Daily caloric intake (in kJ) of Ati-CB1–WT (SD fed, n = 11; HFD fed, n = 21) and Ati-CB1–KO mice (SD fed, n = 17; HFD fed, n = 27) on SD and HFD. (B) Pair-feeding experiment. Body weight curves of Ati-CB1–WT (n = 8) and Ati-CB1–KO (n = 13) on HFD. Tissue NE turnover in EF (C), SF (D), and BAT (E) from Ati-CB1–WT (n = 3) and Ati-CB1–KO mice (n = 4) on HFD. (F) EE in Ati-CB1–WT (n = 16) and Ati-CB1–KO mice (n = 13) on HFD. (G) Ambulatory activity during indirect calorimetry recording in Ati-CB1–WT (n = 16) and Ati-CB1–KO (n = 13) on HFD. (H–J) Gene expression analysis (relative units) of markers for alternatively activated macrophages (Mrc1, Clec10a) in EF, SF, and BAT from Ati-CB1–WT and Ati-CB1–KO mice on HFD (n = 11–12). (K) Protein markers for alternatively activated macrophages (CD206 and CD301) were monitored by flow cytometry in EF from Ati-CB1–WT and Ati-CB1–KO mice on HFD (n = 11–12). (L) Gene expression (relative units) of the catecholamine-synthesizing enzymes (Th, Dbh, Ddc) and alternatively activated macrophage markers (Mrc1, Clec10a) in CD11b+F4/80+-sorted ATMs from WAT of Ati-CB1–WT (n = 6–11) and Ati-CB1–KO (n = 5–11) mice on HFD. Dbh mRNA levels were measured by ddPCR analysis. (M) TH protein levels were measured by flow cytometry in CD11b+F4/80+-sorted ATMs (left) and CD301+ cells (M2 macrophages, right) in WAT from Ati-CB1–WT (n = 9) and Ati-CB1–KO (n = 9) mice on HFD. Data are shown as mean ± SEM. *P < 0.05; #P < 0.01; †P < 0.001, Student’s t test (A, C–E, H–M); 2-way ANOVA (B, F, G).