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. 2017 Oct 3;127(11):4059–4074. doi: 10.1172/JCI94585

Figure 3. Fructose and glucose induce unique lipogenic transcription factors.

Figure 3

(A) mRNA expression and (B) protein levels of SREBP1 transcription factors in whole cell lysates from livers of mice after 10 weeks on diets. (C) Western blots of cytoplasmic and nuclear fractions of truncated active form of (N) SREBP1 and ImageJ quantification of N-SREBP1 protein. (D) mRNA expression of total Chrebp, (E) Chrebp-β isoform, and (F) total protein levels of ChREBP in whole cell liver lysates. (G) Western blots of cytoplasmic and nuclear factions of ChREBP and ImageJ quantification of nuclear fraction. n = 6 mice per group. (H) Immunoprecipitation of ChREBP followed by immunoblot for acetyl-K. 1, Chow+H2O; 2, Chow+Fruct; 3, Chow+Gluc; 4, HFD+H2O; 5, HFD+Fruct; 6, HFD+Gluc, with 3 samples pooled per group. Statistical analysis was performed using 2-way ANOVA with post hoc t tests between the individual groups. #P < 0.05; ##P < 0.01; ####P < 0.0001, compared with Chow+H2O group. *P < 0.05; **P < 0.01; ***P < 0.001, within chow or HFD groups.