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. 2017 Oct 9;127(11):3987–4000. doi: 10.1172/JCI89893

Figure 2. Injection of mast cells induced the formation of neovascular tufts in mast cell–deficient mice.

Figure 2

(A and B) BMCMC but not saline treatment induced the formation of new abnormal blood vessels (white areas) in mast cell–deficient mice with OIR on P17. n = 8 in each group. **P < 0.01 versus saline-injected mast cell–deficient mice, 1-way ANOVA with Tukey’s test. (C) Retinal neovascularization was quantified on P17 by counting the number of neovascular nuclei extending into the vitreous after H&E staining. The number of neovascular nuclei in BMCMC-injected mast cell–deficient pups was comparable to that in WT mice. n = 8 in each group. **P < 0.01 versus saline-injected mast cell–deficient mice, 1-way ANOVA with Tukey’s test. (D and E) Cross-sectional analysis of retinas was performed by H&E (D) or PECAM-1 (E) staining of formalin-fixed paraffin-embedded sections. Arrows indicate endothelial cells that have penetrated into the vitreous space. Results are representative of 3 independent experiments. (F) Normal ERG responses on P19 were seen in age-matched naive WT and saline-injected mast cell–deficient mice, but not in WT or BMCMC-injected mast cell–deficient mice. Scale bars: 500 μm (A); 100 μm (D and E). Results are shown as mean ± SEM of values determined from 3 to 4 independent experiments (B and C).