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. 2017 Oct 31;12(10):e0186614. doi: 10.1371/journal.pone.0186614

Fig 3. TLR9 triggering via ODN CpG 2006 inhibits EBV master lytic gene BZLF1 mRNA expression despite partial protein synthesis inhibition.

Fig 3

First, protein synthesis inhibitors cycloheximide (33 μg/ml) and 4E1RCat (10 μM) were added to Akata cells. After 30 min incubation, cells were treated for 2h with ODN CpG 2006, and finally for 6h with anti-IgG. After a total incubation time of 8.5h, cells were lysed and mRNA was extracted. The BZLF1 mRNA levels relative to HMBS was measured by RT-qPCR and normalized to untreated samples (n = 3). (b) Enlargement of the BZLF1 mRNA levels measured upon incubation with ODN CpG 2006, cycloheximide or 4E1RCat only presented in (a). Data are represented as mean ± SD. Statistics were calculated using the unpaired t test. (*, P<0.05).