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. 2017 Oct 31;12(10):e0186614. doi: 10.1371/journal.pone.0186614

Fig 6. TLR9-induced inhibition of BZLF1 expression is strictly IRAK4-, TLR9- and MyD88-dependent.

Fig 6

CRISPR/Cas9 mutated IRAK4, TLR9 and MyD88 clones were treated for 2h with ODN CpG 2006 prior to lytic reactivation by Ig-crosslinking with anti-IgG for 6h. BZLF1 mRNA expression level normalized to HMBS and relative to untreated cells was measured by RT-qPCR in IRAK4 clones, TLR9 clones and MyD88 clones. Shown is one representative experiment out of three. Data are represented as mean ± SD (n = 3). Statistics were calculated using the unpaired t test. (***, P<0.001; *, P<0.05; n.s., not significant).