Table 2. Sequencing summary of CRISPR/Cas9 IRAK4-, TLR9- and MyD88- clones.
| Clones | DNA sequence changes | Amino acid change |
|---|---|---|
| IRAK4- a3 | c.25inA | p.Thr9AspfsX19 |
| IRAK4- a4 | c.24delA | p.Thr9HisX10 |
| TLR9- b3 | c.2615_2690delCCTTCGTGGTCTTCGACAAAACGCAGAGCGCAGTGGCAGACTGGGTGTACAACGAGCTTCGGGGGCAGCTGGAGGA + c.2692T>C | p.Ala872GlyX73 |
| TLR9- b5 | c.2672inC | p.Leu891ProfsX31 |
| TLR9- b6 1* | c.2672inC | p.Leu891ProfsX31 |
| TLR9- b6 2* | c.2447G>A + c.2655_2682delCTGGGTGTACAACGAGCTTCGGGGGCAG | p.Trp816X |
| MyD88- a3 | c.154_165delGTGCGGACACAG | p.53_56RTAVdel |
| MyD88- a5 | c.161delC | p.Thr54AspfsX46 |
| MyD88- b1 | c.336delG | p.Asp112GlufsX12 |
DNA sequence changes nomenclature is based on the coding DNA sequence.
* TLR9- b6 derives from two different clones whose sequence was determined by TA cloning and sequencing.