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. 2017 Oct 2;3(10):718–727. doi: 10.1021/acsinfecdis.7b00047

Figure 3.

Figure 3

DDD806905 mode of inhibition. (A) LdMetRS biochemical mode of inhibition studies were carried out with reciprocal plots of A650 and substrate concentration revealing that DDD806905 displays competitive inhibition with respect to methionine with a Ki of 18 nM calculated. (B) Mode of inhibition studies also revealed that DDD806905 displays mixed inhibition with respect to ATP with a Ki of 21 nM calculated. Inhibitor concentrations used were as follows: 2 × IC50 (closed circles), 1 × IC50 (open circles), 0.5 × IC50 (closed triangles), and 0 × IC50 (open triangles).