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. Author manuscript; available in PMC: 2017 Oct 31.
Published in final edited form as: Protein Expr Purif. 2012 Dec 13;88(1):98–106. doi: 10.1016/j.pep.2012.11.018

Figure 3. SaPyrC Expression and Purification, Gel Documentation and Analysis.

Figure 3

Samples were loaded in 10 μL amounts in 1× SDS loading dye, and unless noted, samples were taken unaltered from resulting steps described in the methods and diluted 2-fold with 2× SDS loading dye. Lane 1 ladder, lane 2 culture before IPTGA, lane 3 culture after IPTGA, lane 4 crude lysateB, lane 5 supernatantB, lane 6 pelletB, lane 7 flow through (nickel column 1), lane 8 pooled peak (nickel column 1), lane 9 final digestion after 72 hours, lane 10 pooled peak cleaved proteinC (nickel column 2), lane 11 pooled peak cleaved protein gel filtrationC, lane 12 ladder. A, B adjusted to same concentrations C concentrated prior to taking gel sample