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. Author manuscript; available in PMC: 2018 Mar 3.
Published in final edited form as: J Proteome Res. 2017 Feb 3;16(3):1216–1227. doi: 10.1021/acs.jproteome.6b00817

Figure 1. Non-selective kinase affinity reagents and experimental protocol for enrichment/soluble competition and LC-MS/MS analysis of cellular kinases.

Figure 1

a) Non-selective type I (1–3) and type II (4–7) kinase inhibitor analogs used to generate the kinobead affinity matrices in these studies. b) Our kinase inhibitor profiling workflow features microgram-scale affinity enrichment of cellular kinases with kinobeads in SILAC cell lysates, soluble competition, on-bead digest of protein samples, and single-shot nanoLC-MS/MS analysis.