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. 2017 Oct 31;12(10):e0187308. doi: 10.1371/journal.pone.0187308

Fig 3. PIK3C2B plasmid engineering and validation.

Fig 3

A Chromatograms of engineered PIK3C2B vectors B Relative mRNA expression of PIK3C2B after transfection of HEK293 cells (36h) with plasmid constructs. PIK3C2B expression was normalized to housekeeping genes hypoxanthin phosphoribosyltransferase 1 (HPRT) and TATA box binding protein (TBP). Means ± SEM; n = 3 independent experiments C Relative protein expression of PI3KC2β after transfection of HEK293 cells (36h) with plasmid constructs. Protein expression levels were normalized to empty vector EPIK2B. Means ± SEM; n = 3 independent experiments D Expression of exogenous PI3KC2β, visualized with immunofluorescence after transfection of HEK293 cells with plasmid constructs. Staining with DAPI (blue) and MycTag antibody (violet).