Table 1. Models to evaluate the role of HuR in PARPi response.

Table indicates the IC₅₀ values of 1) (CRISPR)-generated PDA cell lines DDR- P MIA PaCa-2 and DDR- D Hs 766T with HuR genetically knocked out [HuR (+/+), HuR (−/−)]; 2) siRNA oligos against the HuR coding region as previously described (17, 20); 3) a small molecule inhibitor, MS-444 (40).

	DDR status	Cell lines	Olaparib			Veliparib			Rucaparib
			IC50 μM	Fold- change	P-value (two- tailed)	IC50 μM	Fold- change	P-value (two- tailed)	IC50 μM	Fold- change	P-value (two- tailed)
CRIPSR knockout	Deficient	HST.HuR(+/+)	8.30	21.8	>0.0001	2.20	2.6	0.0009	4.56	12.6	>0.0001
		HST.HuR(−/−)	0.38			0.85			0.36
	Proficient	MIA.HuR(+/+)	12.52	21.9	>0.0001	12.04	15.5	>0.0001	10.22	20.8	>0.0001
		MIA.HuR(−/−)	0.57			0.76			0.49
siRNA silencing	Deficient	Capan-1 siCon	1.28	3.5	>0.0001	4.25	10.1	>0.0001	10.40	5.3	>0.0001
		Capan-1 siHuR	0.36			0.42			1.95
	Deficient	Hs 766T siCon	8.40	3.8	>0.0001	6.25	2.0	>0.0001	9.25	2.76	>0.0001
		Hs 766T siHuR	2.24			3.12			3.35
	Proficient	MIA PaCa-2 siCon	13.21	6.20	>0.0001	13.32	5.3	>0.0001	15.60	6.24	>0.0001
		MIA PaCa-2 siHuR	2.13			2.50			2.50
	Deficient	Capan-1 (Vehicle)	1.87	3.0	0.0009	1.20	2.1	0.0009	1.65	3.8	>0.0001
		Capan-1 (MS-444)	0.62			0.56			0.43
Small Molecule inhibition (MS-444)	Deficient	Hs 766T (Vehicle)	4.32	2.3	>0.001	3.0	4.1	>0.0001	4.18	3.2	>0.001
		Hs 766T (MS-444)	1.87			0.73			1.31
	Proficient	MIA PaCa-2 (Vehicle)	11.67	4.4	>0.0001	12.56	4.0	>0.0001	11.88	3.2	0.0009
		MIA PaCa-2 (MS-444)	2.68			3.20			3.68