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. 2017 Aug 3;8(48):83370–83383. doi: 10.18632/oncotarget.19848

Figure 3. Alternative splicing in PanIN.

Figure 3

A. Relative quantification of isoform-specific mRNA in pancreatic cell lines. One normal pancreatic cell line (HPDE) and 3 cancer cell lines were assayed for specific isoforms of MUC1, ANXA2, and MYO10 by RT-PCR. Relative isoform mRNA levels after normalization to the corresponding 18S rRNA are shown. B. Total MYO10 mRNA expression is not significantly different in PanIN3 samples compared to normal pancreatic duct samples by RNA-seq. C. MYO10 protein isoform expression by Western blot in cell lines and patient samples. All tested samples are positive for the headless isoform (165 kDa). MiaPaCa2 and A6 pancreatic cancer cell lines are positive for the full isoform (240 kDa). HPNE = normal pancreatic cell line; Panc8.13, MiaPaCa2, A6, Panc1, and Panc10.7 = pancreatic cancer lines; PT78475 and PT77890 = primary pancreatic cancer samples.