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. 2015 May 9;8(48):83457–83468. doi: 10.18632/oncotarget.4054

Figure 4. SH-I-14 induces demethylation and re-expression of tumor suppressor genes.

Figure 4

Cells were treated with SH-I-14 for 48 hr and subjected to DNA methylation assay A., qRT-PCR for mRNA expression B., or western blot analysis C.. A. Genomic DNAs from the cells treated with SH-I-14 were treated with methylation-specific restriction enzymes followed by qRT-PCR to determine the methylation status of each promoter. B. The cDNAs, synthesized from total RNAs of the cells incubated with SH-I-14, were used for qRT-PCR to measure the level of mRNAs. GAPDH level was used to normalize the level of cDNAs. (A~B) *P < 0.05; **P < 0.01; and ***P < 0.001. C. The lysates from the cells treated with SH-I-14 for 48 hr were analyzed by western blot with indicated antibodies. β-actin was used as a loading control.