Figure 4. CRO reduces the transcriptional expression of ENaC α- and γ-subunits.

H441 cells were treated with CRO for 0 to 24 h and then RNA samples were isolated for real-time PCR assays. (A, B) Relative levels of mRNA were calculated as α- or γ-ENaC/GAPDH ratios. ^*P < 0.05, ^**P < 0.01, compared with control; average of 3 experiments for each type of subunit.