Skip to main content
. 2017 Jul 31;8(48):83831–83844. doi: 10.18632/oncotarget.19747

Figure 5. Direct targeting of MCU by miR-340 inhibits motility of and the Warburg effect in MCF7 cells.

Figure 5

(A) Western blots showing MCU expression levels in MCF7 cells. MiR-340 was co-transfected with an MCU–wild-type construct (MCU), an MCU mutant construct in which the target sequence of miR-340 was mutated (MUT), or NC. ATP-5α was used as an internal control. (B) Kinetics of [Ca2+]m in MCF7 cells treated as in (A) according to Rhod-2 fluorescence imaging. (C) Wound-healing (top, middle) and Transwell invasion (bottom) assays in MCF7 cells treated as in (A). (D–G) Glucose uptake, ATP levels, LDH levels, and lactate production in MCF7 cells treated as in (A). The error bars in all the bar graphs represent SD. Statistical significance was determined via one-way analysis of variance followed by pairwise t-tests.*P<0.05; **P< 0.01.