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. 2017 Aug 8;8(48):83872–83889. doi: 10.18632/oncotarget.20040

Figure 12. Silencing of Src with small interfering RNA (siRNA) inhibits expression of fibronectin, collagen 1 and α-SMA in cultured human peritoneal mesothelial cells.

Figure 12

HPMCs were transfected with scrambled siRNA or siRNA specific to Src and incubated for 48 h in DMEM-F12 with 5% fetal bovine serum. (A) Cells were harvested and cell lysates were subject to immunoblot analysis with antibodies to collagen 1, α-SMA, fibronectin, phospho-Src, Src, and GAPDH. (B) Expression levels of indicated proteins were quantified by densitometry and normalized with GAPDH. (C) Expression levels of p-Src and Src were quantified by densitometry and then normalized with Src and GAPDH, respectively. Data are means ± S.E.M. Bars with*are significantly different from controls (P < 0.05).