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. 2017 Aug 3;8(48):83900–83912. doi: 10.18632/oncotarget.19911

Figure 4. SYK Knockdown in OSCC cell lines inhibits cell proliferation, migration, and invasion.

Figure 4

(A): SYK mRNA expression by RT-qPCR analysis for cells transfected by siRNA-SYK (25 nM) and the contrast (siRNA-control) (25 nM) for 24 h. (B): After transfected by siRNA for 48 h, cell lysates were obtained and evaluated for expression of phosphorylated, total SYK, and GAPDH by Western blot. (C): CAL27 cells were seeded in 96-well plate and transfected by siRNA-SYK and siRNA-control. CCK8 assay was used to detect cell viability. (D): Representative images (left) were obtained from the wound healing assay transfected by siRNA-SYK and siRNA-ctrl. Quantitative analysis of the migration was indicated using migration index (right). (E): Representative images (left) and the quantitative analysis (right) indicated the invasion ability of CAL27 cells after transfected by siRNA-SYK and siRNA-control using transwell invasion assay. ** p < 0.01; *** p < 0.001.