Figure 2. LiCl reduced inflammatory cytokine expression, including TNF-α, IL-1β, and IL-6.

Protein levels of TNF-α (A), IL-1β (B), and IL-6 (C) were obtained from the supernatant of cultured mice calvariae, then analyzed by ELISA (^*P<0.05 and ^**P<0.01). (D) Immunohistochemical staining for TNF-α, IL-1β, and IL-6 in the mouse calvarial osteolysis model (brown, indicated by the arrows, Scar bar = 100 μm).