Figure 4. LiCl inhibited osteoclastic bone resorption and impaired the formation of characteristic osteoclast rings.

(A) Equal number of cells were seeded onto an Osteo Assay Plate and cultured in induction medium with various LiCl concentrations until mature osteoclasts formed. (B) The area of bone resorption was quantitated using Image J 6.0 software. (^*P<0.05 and ^**P<0.01). (C) After pretreatment with various concentrations of LiCl for 4h, BMMs were cultured in induction medium for 5 days. The cells were then prepared for F-actin staining.