Figure 5. LiCl inhibited RANKL-induced osteoclast gene expression, including NFATc1, CTSK, Sema-4A, and Oscar.

After pretreatment with various LiCl concentrations (0.2, 1, and 5 mM) or with 5 mM LiCl for 4h, BMMs were cultured in induction medium for 5 days (A) or for the indicated time (1, 3, 5 day) (B). Total RNA was extracted using an RNeasy mini kit. Osteoclast gene expression was determined by qRT-PCR. (^**P<0.05 and ^**P<0.01).