Figure 7. LiCl suppressed the RANKL-stimulated activation of NF-κB without affecting the PI3k/Akt and MAPK signaling pathways.

(A & C) After exposure or no exposure to 5mM LiCl for 4h, 50 ng/ml RANKL was added to stimulate RAW264.7 cells for 0, 5, 15, 30, or 60 min. The cells were then collected and lysed for western blot assay. (B & D) The grey levels corresponding to Ser32-IκBα, Ser536-p65, S473-AKT1, Thr202/Tyr204-ERK, Thr183/Tyr185-JNK, and Thr180/Tyr182-p38 phosphorylation were analyzed using Image J 6.0 software (^*P<0.05 and ^**P<0.01).