Skip to main content
. 2017 Sep 19;8(48):84006–84018. doi: 10.18632/oncotarget.21061

Figure 6. Low levels of NEO1 are related with BCC aggressiveness.

Figure 6

(A–E) Subtypes of sporadic human BCC samples were assessed by histomorphological analysis. mRNA levels of both NEO1 (A) and GLI1 (B) of aggressive BCC subtypes (n = 10) are significantly lower than levels of non-aggressive BCC (n = 22). Data is represented as mean ± SEM with *p < 0.05, **p< 0.01; according to Mann-Whitney test. (C) Representative WB membrane of NEO1 expression in non-aggressive and aggressive BCC subtypes, ACTB is shown as a loading control. (D) The levels of NEO1 in non-aggressive BCC is higher than in aggressive BCC subtypes. Data correspond to n = 8 for non-aggressive BCC samples and n = 4 for aggressive BCC samples and are normalized against ACTB expression. *p < 0.05, according to Mann-Whitney test. (E) H&E of representative BCC biopsies (left column, bar = 250 μm) show histological differences between aggressive and non-aggressive BCC subtypes. IF for NEO1 (green) and PCNA (red) of tumor nodules of these samples show strong staining on non-aggressive tumor cells and dimmer staining for aggressive tumor cells. DAPI (blue) was used for nuclei staining (bar = 50 μm). Negative controls are shown as insets.