Figure 3. DFMO and Trimer PTI co-treatment increases cytotoxic T-cell activity and promotes macrophage infiltration of the tumor.

(A) The frequency of IFN-γ producing T-cells was measured by the ELISpot assay as IFN-γ spot forming units (SFU) per million spleen cells. (B) The number of F4/80⁺ cells per million B16F10-sTAC melanoma cells from either vehicle treated mice or mice co-treated with DFMO and Trimer PTI. Representative images of B16F10-sTAC tumor sections from mice treated with vehicle (C) or DFMO and Trimer PTI (D) and stained for F4/80⁺ macrophages. n = 5-10 mice per group; ^* = p ≤ 0.05 and # = p ≤ 0.01 compared to vehicle treated mice.