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. 2017 Sep 6;8(48):84276–84284. doi: 10.18632/oncotarget.20685

Figure 2. Overexpression of Pkm1 and Pkm2 enhanced pluripotent genes in ESCs.

Figure 2

(A) Morphology of the ES cell lines with Dox inducible overexpression of Pkm1 (upper) or Pkm2 (lower). EGFP indicating the activity of distal enhancer of endogenous Oct4. mKO exhibiting the ectopic expression of Pkm1 or Pkm2 under Dox induction. (B) Western blot showing the overexpression of Pkm1 and Pkm2 in established ES cell lines. (C) Real-time PCR examining the expression changes of Nanog, Eras and Rex1 under Pkm1 or Pkm2 overexpression in ESCs. (D) Expression changes of Oct4, Fgf5, Afp and Gata6 on Day 0, Day 3 and Day 6 during the differentiation of Pkm1 (upper) or Pkm2 (lower) overexpression ES cell lines. Scale bars represent 200 μm. Data represented as mean ± SD; n = 3.