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. 2017 Sep 15;8(48):84285–84299. doi: 10.18632/oncotarget.20894

Figure 3. ATP1B3 expression was knocked down by transfection with ATP1B3-siRNA in SGC-7901 cells.

Figure 3

(A) Representative photomicrographs of bright fluorescence in SGC-7901 cells were obtained at a magnification of 100x. At 48 h after infection, approximately 50% of the cells expressed FAM at the time points as determined by fluorescence microscopy. (B) Western Blot measured ATP1B3 protein expression in SGC-7901 cells and in cells transfected with siRNA1, siRNA2, siRNA3, or si-NC. It showed that ATP1B3 siRNA1 was the most efficient knockdown sequence compared with the other siRNAs. *P<0.05 compared with SGC-7901 and Control cells. (C) qRT-PCR assay analysis of ATP1B3 mRNA knockdown efficiency using siRNA1, siRNA2, siRNA3 and si-NC in SGC-7901 cells. *P<0.0.1 vs. Control. All of three siRNA fragments demonstrated good knockdown efficiency with respect to ATP1B3 mRNA, and siRNA1 had the highest interference efficiency from the average value.