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Conditioned medium of HEK-EBNA cells transfected with plasmids carrying different SERPINC1 mutations was incubated for 5 min with human thrombin and UFH. Antithrombin (AT) glycoforms (α and β) and thrombin-antithrombin complex (TAT) pointed by arrows were detected after SDS-PAGE by Western blot using a polyclonal antibody against human antithrombin.
