Fig 10. Restoration of Bbs8 expression by tamoxifen injections between P9-P15 provides full rescue of retinal phenotypes.

Mice were injected with tamoxifen between P9-P15. (A) BBS8 protein levels in one month old Bbs8^gt/gt; Flp^- and Bbs8^gt/gt; Flp⁺ mice with or without tamoxifen treatment. (B) Quantification of BBS8 band intensities in the western blot in (A) shows BBS8 proteins in rescued Bbs8^gt/gt; Flp⁺ mice recovered to greater than 65% of BBS8 levels in wild type mice. (C) Electroretinogram (ERG) function of treated Bbs8^gt/gt; Flp⁺ at one month of age. (D) STX3 localization in treated Bbs8^gt/gt; Flp⁺ mice. (E) Morphological rescue of inner and outer segment thickness in Bbs8^gt/gt; Flp⁺ mice. Perdurance of rescue is demonstrated by ERG (F), STX3 localization (G), and histology (H) at 4 months after the last tamoxifen injection (4MP-TMX). P values for one-way ANOVA followed by post-hoc Tukey’s test are shown: * denotes p<0.05, ** denotes p < 0.01, *** denotes p < 0.005, **** denotes p < 0.001, ***** denotes p < 0.0005, ****** denotes p < 0.0001. Scale bar: 50 μm.