Fig 12. BBS8 protein levels in the eye during and after tamoxifen injections at P9, P12, and P15.

(A) BBS8 protein levels in whole eye lysates of Bbs8^gt/gt; Flp⁺ mice and Bbs8^wt/wt; Flp⁺ or Flp^- mice. For the P11 time point, mice were injected with tamoxifen at P9 and sacrificed 2 days later. For the P14 time point, mice were injected at P9 and P12 and sacrificed 2 days later. For the P17 time point, mice were injected with tamoxifen at P9, 12, and 15 and sacrificed 2 days later. For the 1 month time point, mice were injected at P9, 12, and 15 and sacrificed 2 weeks later. (B) Percent BBS8 protein levels in Bbs8^gt/gt; Flp⁺ mice compared to their respective wild type controls at those time points. BBS8 protein levels in the Western blot in panel (A) are quantified and normalized to actin. Normalized BBS8 protein levels of rescued mice are divided by normalized BBS8 protein levels of wild type mice to derive the ratio of BBS8 protein levels.