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. 2017 Oct 19;13(10):e1007057. doi: 10.1371/journal.pgen.1007057

Table 1. List of genotyping primers.

PCR Assay Primers Composition Sequences Product Size
BBS8GT F 33% TGGCAAAGGTATGTGCTTCA WT 257bp
GT 168bp
r1 33% GTTGCGCCATCTCATAATAACTA
r2 33% ACATACGCAGACAGTGTCAAGCT
Flp M 20% TTATGTAACGCGGAACTCCA WT 603bp
FLP 309bp
C 20% AAAGTCGCTCTGAGTTGTTAT
W 60% GGAGCGGGAGAAATGGATATG
BBS8GN f1 33% TATCATCTTTGTAGGACGAAGCT WT 193bp
NULL 368bp
f2 33% AGAGTCATGTTAGAACTCCAAGT
R 33% TGTCGGCTGCTGTCTGGTTGAGT
BBS8MT f1 33% AGTTATTATGAGATGGCGCAACG NULL/EXCISED 368bp
FLOXED 250bp
f2 33% AGAGTCATGTTAGAACTCCAAGT
R 33% TGTCGGCTGCTGTCTGGTTGAGT
Cre cre-f 10% GGCCAGCTAAACATGCTTCATC Ctrl 397bp
Cre 250bp
cre-r 10% CCTGATCCTGGCAATTTCGGCT
ctrl-f 40% CTGTACACCACGGTCTCCAAT
ctrl-r 40% ATAATGCTGGGTGAAATGCAG