Table 1. List of genotyping primers.
PCR Assay | Primers | Composition | Sequences | Product Size |
---|---|---|---|---|
BBS8GT | F | 33% | TGGCAAAGGTATGTGCTTCA | WT 257bp GT 168bp |
r1 | 33% | GTTGCGCCATCTCATAATAACTA | ||
r2 | 33% | ACATACGCAGACAGTGTCAAGCT | ||
Flp | M | 20% | TTATGTAACGCGGAACTCCA | WT 603bp FLP 309bp |
C | 20% | AAAGTCGCTCTGAGTTGTTAT | ||
W | 60% | GGAGCGGGAGAAATGGATATG | ||
BBS8GN | f1 | 33% | TATCATCTTTGTAGGACGAAGCT | WT 193bp NULL 368bp |
f2 | 33% | AGAGTCATGTTAGAACTCCAAGT | ||
R | 33% | TGTCGGCTGCTGTCTGGTTGAGT | ||
BBS8MT | f1 | 33% | AGTTATTATGAGATGGCGCAACG | NULL/EXCISED 368bp FLOXED 250bp |
f2 | 33% | AGAGTCATGTTAGAACTCCAAGT | ||
R | 33% | TGTCGGCTGCTGTCTGGTTGAGT | ||
Cre | cre-f | 10% | GGCCAGCTAAACATGCTTCATC | Ctrl 397bp Cre 250bp |
cre-r | 10% | CCTGATCCTGGCAATTTCGGCT | ||
ctrl-f | 40% | CTGTACACCACGGTCTCCAAT | ||
ctrl-r | 40% | ATAATGCTGGGTGAAATGCAG |