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. Author manuscript; available in PMC: 2017 Oct 31.
Published in final edited form as: Handb Exp Pharmacol. 2007;(179):313–328. doi: 10.1007/978-3-540-34891-7_19

Fig. 1.

Fig. 1

a–d Development of heterologous and endogenous TRPM7 currents. a Representative development of heterologous TRPM7 currents in overexpressing HEK-293 cells where free Mg2+]i was kept at 800 μM and MgATP was omitted (n = 6). Data points correspond to average and normalized current amplitudes measured at −80 mV (closed circles) and +80 mV (open circles) and plotted as a function of time. Note that TRPM7 develops immediately. b Current–voltage (I–V) relationship of heterologous TRPM7 extracted from an example cell at 200 s. c Representative development of endogenous TRPM7-like MagNuM currents measured in RBL-2H3 cells. Average inward (closed circles) and outward (open circles) currents at −80 mV and +80 mV, respectively (n = 6), recorded under conditions that suppress ICRAC and support MagNuM activation (omission of MgATP, free [Mg2+]i = 760 μM, free [Ca2+]i = 100 nM). Note that MagNuM starts to develop around 80 s into the experiment. d Representative I–V relationship of endogenous TRPM7-like MagNuM extracted at 300 s after whole-cell establishment