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. 2017 Oct 27;8:1399. doi: 10.3389/fimmu.2017.01399

Figure 2.

Figure 2

Invariant natural killer T (iNKT) cells from c-Maf knockout (KO)-reconstituted mice do not produce IL-17A upon α-galactosylceramide (α-GalCer) stimulation. (A) Total splenocytes were isolated from wild-type (WT) or c-Maf KO reconstituted mice and stained with PBS-57-loaded CD1d tetramer and monoclonal antibodies (mAbs) specific for TCR-β, CD4, and NK1.1. The percentages of iNKT cells among splenocytes and the percentage of the four subsets of iNKT cells (defined by NK1.1 and CD4) among total iNKT cells are determined by flow cytometry. Representative FACS plots are shown. NS, not significant, determined by unpaired t-test, two-tailed (n = 3). (B) The splenocytes were also stimulated with α-GalCer (100 ng/ml) for 4 days in vitro. The levels of IFN-γ, IL-4, IL-17A, and IL-10 in the culture supernatants were measured by ELISA. NS, not significant, *P < 0.05 determined by unpaired t-test, two-tailed (n = 3).