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. 2017 Oct 27;8:1399. doi: 10.3389/fimmu.2017.01399

Figure 4.

Figure 4

Splenic and lymph node (LN) c-Maf knockout (KO) invariant natural killer T (iNKT) 17 cells express less IL-17A than wild-type (WT) counterparts. Splenic (A) and LN (B) iNKT cells from WT or c-Maf KO reconstituted mice expanded in vitro with α-galactosylceramide (α-GalCer) for 5 days, sorted, and stimulated with anti-CD3/anti-CD28 plus IL-6/TGF-β/IL-1β (iNKT17-skewing conditions). The cells were restimulated with PMA/ionomycin, and the expression of indicated cytokines and transcription factors was examined with intracellular cytokine staining and ELISA (A,B), or quantitative PCR (C). Representative FACS plots and cumulative results are shown. NS, not significant, *P < 0.05; **P < 0.01; ***P < 0.001 determined by unpaired t-test, two-tailed (n = 3). (C) iNKT17-related mRNA profiles were measured by quantitative PCR. The expression of mRNA in each sample was normalized against the level of β-actin. The data shown are from three independent experiments, and the values from WT are arbitrarily set as 1.0. UD, undetectable.