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. 2017 Oct 27;8:282. doi: 10.3389/fendo.2017.00282

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Copyright © 2017 Bauman, Yin, Gore and Wu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of age and estradiol on mRNA expression of Gnrh1 and Gnrhr. (A) The expression of Gnrh1 mRNA was analyzed within the medial preoptic area (mPOA). (B) The expression of Gnrhr mRNA was analyzed within the pituitary (PIT). Comparisons of age were made between groups 1–4 (analysis 1), duration of hormone treatment between groups 3–6 (analysis 2), and timing of hormone treatment between groups 5–8 (analysis 3). All comparisons were made as described in Section “Materials and Methods.” Data shown are mean ± SEM (n = 6–7). *p < 0.05 (analysis 1); #p < 0.05 (analysis 2); †p < 0.05 (analysis 3).