Figure 5.

Flow cytometry-based EC₅₀ measurements with mean fluorescence intensity (MFI) for the IgGs binding to cell surface-expressed H1N1 CA/09 and H5N1 VN/04 HA trimers on 293 T cells. (a),(b) The IgGs were reformatted from the selected anti-HA trimer scFvs as shown in Fig. 3. The experimental details are described in Methods. The CDR sequences of these IgGs are shown in Supplementary Table S3, and the numerical values of the EC₅₀’s and the maximal MFI are listed in Supplementary Table S4. The experimental details are described in Methods.