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. 2017 Oct 31;7:14438. doi: 10.1038/s41598-017-14832-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Homozygous osnramp5 mutants induced by CRISPR/Cas9. (a) DNA sequence alignments for the 5 homozygous osnramp5 mutants identified in the T₁ generation, together with a wild-type (WT) control. The numbers on the right side are the sizes of the indels, with “−” and “+” showing deletion and insertion of nucleotides involved, respectively. (b) Deduced OsNramp5 amino acid sequence alignments for the 5 homozygous mutants and WT. Each of the mutant alleles codes for truncated and disrupted OsNramp5 proteins.