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. 2017 Oct 31;7:14438. doi: 10.1038/s41598-017-14832-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Concentrations of Cd and Mn in shoots and roots at different Cd levels. Three osnramp5 mutant lines (HZ-6-4-6, HZ-7-3-2, HZ-7-3-12) and WT plants (HZ) were grown in normal nutrient solution for 2 weeks and then transferred to the nutrient solution containing 0.5 or 2.5 µM Cd for another 2 weeks. The shoots and roots were harvested and subjected to mineral analysis by inductively coupled plasma optical emission spectrometry (ICP-OES). (a and b) Cd concentrations in the shoots (a) and roots (b). (c and d) Mn concentrations in the shoots (c) and roots (d). DW, dry weight. Data are means ± SD of three biological replicates, and three plants were mixed in one replication for metal determination. Two asterisks indicate statistically significant difference in comparison to WT at P < 0.01 by Student’s t test.