Fig. 1.

Analysis of Verticillium primers specificity. Primers listed in Table 2 of a V. longisporum, b V. dahliae, c V. albo-atrum, d V. tricorpus were tested against the other Verticillium species. Amplification was observed only on-target Verticillium species. No amplification on off-target species and on mock samples was observed. Melt curve analysis shows amplification of a single fragment. Two technical replicates were used