A and C, effect of cysteine‐modifying reagents on 8A‐VFP/8C‐mCh (A) and 8A‐VFP/8D‐mCh (C) co‐expressing oocytes. One‐sample t test with respect to control value 1. In A, Chl‐T, P = 3 × 10−8
** as in Fig. 4; MMTS, P = 0.25, n = 4; MTSET, P = 0.050, n = 3; MTSES, P = 0.004**, n = 3. In C, Chl‐T, P = 0.0004** as in Fig. 5; MMTS, P = 0.0013**, n = 4; MTSET, P = 0.03*, n = 3; MTSES, P = 0.06, n = 3. B and D, effect of subsequent application of chloramine‐T in the presence of cysteine‐modifying agents in the bath. No significant alteration of chloramine‐T‐induced reduction of currents was observed (ANOVA: P = 0.34 (B); P = 0.23 (D); n ≥ 3 each, error bars indicate SEM). Note the different time and current scales in the various panels. [Color figure can be viewed at wileyonlinelibrary.com]